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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

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Overexpression of the gene is noticed in several tumors, which results in chromosome instability, and so might contribute to tumorigenesis. This protein was uncovered to associate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a essential job in selling mobile-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, are already described. Two additional splice variants have been noted but detailed nucleotide sequence information and facts is not yet readily available.

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Blocking peptides are peptides that bind exclusively towards the target antibody and block antibody binding. These peptide generally consists of the epitope acknowledged from the antibody.

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This manual outlines further feasible optimization of cellular and immunoassay parameters to ensure the best possible effects are...

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This manual outlines additional achievable optimization of mobile and immunoassay parameters to be sure the best possible success are...

Antibodies bound to the blocking peptide now not bind for the epitope over the goal protein. By evaluating the staining through the blocked antibody versus the antibody alone, one can see which staining is certain.

Goal Data Cyclin E belongs for the very conserved cyclin spouse and children, whose associates are characterized by a dramatic periodicity in protein abundance from the cell cycle. Cyclins functionality as regulators of CDK kinases. Various cyclins exhibit distinctive expression and degradation styles which add to your temporal coordination of every mitotic celebration. Cyclin E kinds a fancy with and functions to be a regulatory subunit of CDK2, whose exercise is needed for cell cycle G1/S changeover. This protein accumulates for the G1-S stage boundary and is degraded as cells development by means of S section.

The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...

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